O-007 Association of successful embryo development and pregnancy outcome with redox state as measured by human non-mercaptalbumin in embryo culture medium

Abstract Study question Is the redox state in embryo culture medium, as measured by oxidized albumin, associated with successful development and pregnancy outcome? Summary answer The level of albumin medium was most important variable predicting blastocyst formation andwas miscarriage after transfer. What is known already Human serum (HSA), abundant protein plasma, exerts antioxidant activities against oxidative damage. HSA exists human non-mercaptalbumin (HNA) reduced mercaptalbumin. HNA attracting attention because its novel role a marker reflecting state, levels are to increase stress diseases such kidney disease, diabetes, liver disease or Parkinson's disease. On other hand, IVF, supplemented source, but no previous studies have examined relationship between IVF outcomes. design, size, duration We followed outcomes for 40 cycles single transfer out retrospective study, which enrolled total 173 embryos cultured stage from 91 patients who underwent IVF/ICSI cycle February 2018 July 2018. Participants/materials, setting, methods Prior using culture, assessed level. level, patient age, oocyte maturity were analyzed factors on day 5 6 fertilization, machine learning model developed Random Forest (RF) algorithm predict formation. also whether made difference Main results chance median %HNA 89.36% (range, 80.12% 94.84%), much higher than value blood healthy humans, (approximately 25%). Blastocyst observed 41.04% (71/173) embryos. In both univariate multivariate analyses, lower (p = 0.001), younger age < use standard 0.007). A prediction RF four (%HNA, fertilizationmethod, maturation stage). 70% samples (training set, n 121) validated remaining testing set (n 52) produced an area under curve 0.761, where formation, age. significantly group that resulted (93.15% vs. 89.28%, P 0.0498). Limitations, reasons caution This study conducted G-TL only. Further needed elucidate association development, success rate commonly used media. Wider implications findings supposed mimic body environment, showed high compared itself; it suggesting may cause unsuccessful live birth rate. Controlling oxidation help create more appropriate environment. Trial registration number not applicable